Genetic Polymorphisms of Cytochrome P450 2D14 (CYP2D14) Gene in Japanese Black Cattle and Holstein-Friesian Cattle

Two types of cytochrome P450 2D14 (CYP2D14) gene deletions (GD1 and GD2) in Japanese Black cattle has been reported by us. In this study, we determined the frequency of cytochrome P450 2D14 (CYP2D14) genetic polymorphisms in 48 Japanese Black (JB) and 48 Holstein-Friesian (HF) cattle using polymerase chain reaction (PCR)-direct sequencing. Two types of CYP2D14 gene deletion variants, GD1 and GD2, were observed 58% in JB cattle, but only 8% of HF cattle. Genotype frequencies of CYP2D14 gene (105 C>A) also were assessed, but the AA substitution was not detected. The results of CYP2D14 gene variation study in cattle show that CYP2D14 GD allele may be an important mutation relevant to an enzyme defect in JB cattle because of high frequency of gene deletion variants. Genetic Polymorphisms of Cytochrome P450 2D14 (CYP2D14) Gene in Japanese Black Cattle and Holstein-Friesian Cattle